Journal: Clinical Oral Investigations

Article Title: Influence of cold atmospheric plasma on dental implant materials — an in vitro analysis

doi: 10.1007/s00784-021-04277-w

Figure Lengend Snippet: Determination of cell proliferation by XTT assay applying CAP at various durations. A Proliferation of MG-63 cells after CAP application with 60 s/120 s (intensity: 18 kV); B proliferation of HGF-1 cells after CAP application with 60 /120 s (plasma device intensity: 18 kV); columns and error bars represent the mean and SEM of measured specific absorbance (SA)/(OD blanked 450–690 nm). Mean ± SEM ( n = 6); *significant ( p < 0.05); **** significant ( p < 0.0001) difference between groups

Article Snippet: Human osteoblast-like cells (MG-63) (ATCC, CRL-1427TM) (Sigma‐Aldrich, Taufkirchen, Germany) or Human Gingival Fibroblasts (HGF-1) (330703HPL; CLS Cell Lines Service GmbH, Eppelheim, Germany) were cultured in Dulbecco’s modified essential medium (DMEM, Invitrogen, Germany) supplemented with 10% fetal bovine serum (FBS, Invitrogen), 100 unit’s penicillin, and 100 μg/mL streptomycin (Invitrogen) at 37 °C in a humidified atmosphere of 5% CO 2 and 95% humidity.

Techniques: XTT Assay

Journal: Clinical Oral Investigations

Article Title: Influence of cold atmospheric plasma on dental implant materials — an in vitro analysis

doi: 10.1007/s00784-021-04277-w

Figure Lengend Snippet: Scanning electron microscopy/fluorescence microscopy detecting adhesion assay on titanium/zirconia specimens. Different cell types (MG-63) and (HGF-1) (columns); coated/uncoated implant surface materials (rows). Detailed images showing cell adhesion of cells using high magnification (× 2000). Fluorescence microscopy revealing staining of cell nuclei with DAPI (blue) and the cytoskeleton using phalloidin staining (green)

Article Snippet: Human osteoblast-like cells (MG-63) (ATCC, CRL-1427TM) (Sigma‐Aldrich, Taufkirchen, Germany) or Human Gingival Fibroblasts (HGF-1) (330703HPL; CLS Cell Lines Service GmbH, Eppelheim, Germany) were cultured in Dulbecco’s modified essential medium (DMEM, Invitrogen, Germany) supplemented with 10% fetal bovine serum (FBS, Invitrogen), 100 unit’s penicillin, and 100 μg/mL streptomycin (Invitrogen) at 37 °C in a humidified atmosphere of 5% CO 2 and 95% humidity.

Techniques: Electron Microscopy, Fluorescence, Microscopy, Cell Adhesion Assay, Staining

Journal: Journal of pharmacological sciences

Article Title: Development of multifunctional pyrrole-imidazole polyamides that increase hepatocyte growth factor and suppress transforming growth factor-β1.

doi: 10.1016/j.jphs.2023.11.001

Figure Lengend Snippet: Fig. 4. The effects of pyrrole-imidazole (PI) polyamides (HGF-2 and HGF-4) on the expression of transforming growth factor-β1 (TGF-β1) mRNA in cultured human dermal fibroblasts (HDF) cells. Cells were incubated with HGF-2 and HGF-4 (10−11-10−8 M) or mismatch polyamide for 15 h in the presence or absence of 10−7 M phorbol 12-myristate 13-acetate (PMA) for 12 h. The TGF-β1 mRNA expression was evaluated by real-time PCR in comparison to 18S rRNA. Data are the mean ± SEM (n = 6 for mRNA expression). #P < 0.05 vs. without PMA, *P < 0.05 vs. with PMA without PI polyamides.

Article Snippet: The primary antibodies used were TGF-β1 (1:500; Y241, Yanaihara Institute Inc, Shizuoka, Japan) and HGF (1:500; MAB294, R&D Systems, Minneapolis, MN).

Techniques: Expressing, Cell Culture, Incubation, Real-time Polymerase Chain Reaction, Comparison

Journal: Journal of pharmacological sciences

Article Title: Development of multifunctional pyrrole-imidazole polyamides that increase hepatocyte growth factor and suppress transforming growth factor-β1.

doi: 10.1016/j.jphs.2023.11.001

Figure Lengend Snippet: Fig. 5. The effects of pyrrole-imidazole (PI) polyamides (HGF-2 and HGF-4) on the expression of transforming growth factor-β1 (TGF-β1) protein in cultured human dermal fibroblasts (HDF) cells. Cells were incubated with HGF-2 and HGF-4 (10−11-10−8 M) or mismatch polyamide for 15 h in the presence or absence of 10−7 M phorbol 12-myristate 13-acetate (PMA) for 12 h. (a) The TGF-β1 protein expression was evaluated by a Western blot analysis and normalized to β-actin. (b) Data are the mean ± SEM (n = 3). ##P < 0.01 vs. without PMA, **P < 0.01 vs. with PMA without PI polyamides.

Article Snippet: The primary antibodies used were TGF-β1 (1:500; Y241, Yanaihara Institute Inc, Shizuoka, Japan) and HGF (1:500; MAB294, R&D Systems, Minneapolis, MN).

Techniques: Expressing, Cell Culture, Incubation, Western Blot

Journal: Journal of pharmacological sciences

Article Title: Development of multifunctional pyrrole-imidazole polyamides that increase hepatocyte growth factor and suppress transforming growth factor-β1.

doi: 10.1016/j.jphs.2023.11.001

Figure Lengend Snippet: Fig. 6. The effects of pyrrole-imidazole (PI) polyamides (HGF-2 and HGF-4) on the expression of transforming growth factor-β1 (TGF-β1) protein in cultured human dermal fibroblasts (HDF) cells with HGF siRNA. Cells were preincubated with HGF siRNA or negative control (NC) siRNA for 24 h and then incubated with or without 10−11-10−9 M HGF PI polyamides (HGF-2 and HGF-4) for 15 h in the presence of 10-7 M phorbol 12-myristate 13-acetate (PMA) for 12 h. The TGF-β1 protein expression was evaluated by a Western blot analysis and normalized to β-actin. Data are the mean ± SEM, n = 3. #P < 0.05 vs. without PMA, *P < 0.05, **P < 0.01vs. with PMA + HGF siRNA without PI polyamides.

Article Snippet: The primary antibodies used were TGF-β1 (1:500; Y241, Yanaihara Institute Inc, Shizuoka, Japan) and HGF (1:500; MAB294, R&D Systems, Minneapolis, MN).

Techniques: Expressing, Cell Culture, Negative Control, Incubation, Western Blot